Combinatorial 2-Positional Scan

Combinational 2- Positional Scan

Positional scanning is an important tool for peptide sequence optimization. Two position combinatorial scanning identifies amino acids of interest at two given positions and substitutes them with all other natural amino acids one at a time. Increases in activity will identify the preferred amino acid residues at these positions.

 

Special Instructions

Enter your original sequence below as well as the position of the two amino acids to be substituted, counting from the N-Terminus. A total of 20 x 20 = 400 sequences will be generated.

Peptide Sequence (25 amino acids max) 3 letter converter
Position of residue(s) to be replaced (from Nterm)
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