Overlapping

Overlapping

Overlapping peptide libraries are ideal for T-cell epitope searching, because T cell epitopes are by nature short linear peptides from the primary protein sequence. They are also appropriate for scanning the primary sequence of proteins for linear, or “continuous”, B-cell (antibody-defined) epitopes.

 

Special Instructions

Library generation process is defined by two parameters, peptide length and offset number, which reflects the degree of overlapping. The second last peptide in each set (the 'orphan') is often shorter than the other peptides as it lies at the C-Terminus. The last peptide included in the set represents the orphan sequence plus one or more N-Terminal residues to bring it to the correct length.

 

General rules for library design can be found here. For assistance in designing a library for your particular application, please contact us directly.

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